The membrane with transferred proteins is then probed with a. After SDS-PAGE, a membrane is placed on the gel, to which the separated proteins in the gel are electrophoretically transferred. Interpretation of the WB band pattern in combination with clinical features may be occasionally useful in predicting the stage of HIV infection. In Western blotting (WB), target proteins are transferred to a hydrophobic membrane after SDS-PAGE and detected using specific antibodies. Correlation of CD4 cell counts with the various uncommon band patterns showed that only 5.56% (4/72) had counts in the 200-500 cells/microl range, whereas 45.83% and 48.61% had counts of 500 cells/microl respectively. On classifying the WB reactive cases by the WHO clinical staging system, 38.45% (564/1467) were in Stage 1, 47.99% (704/1467) in stages 2 and 3 and 13.56% in stage 4. Of all individual bands, p31 band was the most frequently missing one, absent in 7.09% cases. Reactivity with all the bands was the most commonly observed WB pattern, occurring in 92.91% (1363/1467) of cases, whereas the other 7.09% showed uncommon band patterns. For the interpretation of the WB results in this study, the guidelines recommended by NACO, India were followed. We performed a retrospective study with 1,467 HIV-1 infected cases confirmed by WB test between January 2002 to July 2005, with the objective of mapping different band patterns of western blot results and determining whether the presence or absence of certain bands was associated with any specific stage of HIV infection. Mapping different band patterns of Western blot results and correlating them with stages of HIV infection. ![]() Although the overall sensitivity and specificity of the western blot (WB) test for detection of antibodies to various viral proteins is high, there has been a substantial difference in the timing of the appearance of antibody bands and their intensities during different stages of HIV infection.
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